【IF2.527-审稿快适合毕业-版面可免期刊】基于网络药理学揭示肉桂抗寒生热机理

【写在前面】：本期推荐的是由沈阳药科大学等团队近日3月份刚发表于Fitoterapia（IF2.527）的一篇文章，基于网络药理学的研究揭示了肉桂的抗寒性和生热机理。

【期刊简介】

 图片源自杂志官网

 杂志主页链接

（网址https://www.journals.elsevier.com/fitoterapia/）

【接收范围及审稿周期】

     Fitoterapia是一本专门针对药用植物和植物来源的生物活性天然产物的杂志。 它发布了七个主要领域的原创文章：

  1.药用植物有效成分的表征

  2.开发生物活性植物提取物和天然产物的标准化方法

  3.鉴定植物提取物中的生物活性

  4.确定植物提取物的目标和活性机制

  5.药用植物生物量的产生和基因组学表征

  6.植物来源的生物活性天然产物的化学和生物化学

  7.对药用植物的历史，临床和法律状况进行严格审查，并就专题问题进行说明。

     选择性OA期刊，平均0.9周给一审结果，审稿周期一般为1~2个月。版面费$ 3300，发展中国家免费。

【题目及作者信息】

  Network pharmacology-based research uncovers cold resistance and thermogenesis mechanism of Cinnamomum cassia

【摘要部分】
Background

Cinnamomum cassia (L.) J.Presl (Cinnamon) was known as a kind of hot herb, improved circulation and warmed the body. However, the active components and mechanisms of dispelling cold remain unknown.

Methods

The effects of several Chinese herbs on thermogenesis were evaluated on body temperature and activation of brown adipose tissue. After confirming the effect, the components of cinnamon were identified using HPLC-Q-TOF/MS and screened with databases. The targets of components were obtained with TCMSP, SymMap, Swiss and STITCH databases. Thermogenesis genes were predicted with DisGeNET and GeneCards databases. The protein-protein interaction network was constructed with Cytoscape 3.7.1 software. GO enrichment analysis was accomplished with STRING databases. KEGG pathway analysis was established with Omicshare tools. The top 20 targets for four compounds were obtained according to the number of edges of PPI network. In addition, the network results were verified with experimental research for the effects of extracts and major compounds.

Results

Cinnamon extract significantly upregulated the body temperature during cold exposure.121 components were identified in HPLC-Q-TOF/MS. Among them, 60 compounds were included in the databases. 116 targets were obtained for the compounds, and 41 genes were related to thermogenesis. The network results revealed that 27 active ingredients and 39 target genes. Through the KEGG analysis, the top 3 pathways were PPAR signaling pathway, AMPK signaling pathway, thermogenesis pathway. The thermogenic protein PPARγ, UCP1 and PGC1-α was included in the critical targets of four major compounds. The three major compounds increased the lipid consumption and activated the brown adipocyte. They also upregulated the expression of UCP1, PGC1-α and pHSL, especially 2-methoxycinnamaldehyde was confirmed the effect for the first time. Furthermore, cinnamaldehyde and cinnamon extract activated the expression of TRPA1 on DRG cells.

Conclusion

The mechanisms of cinnamon on cold resistance were investigated with network pharmacology and experiment validation. This work provided research direction to support the traditional applications of thermogenesis.

背景

肉桂被称为一种热草药，可改善血液循环并温暖人体。然而，有效成分和消除寒气的机制仍然未知。

方法

通过体温和棕色脂肪组织的活化来评估几种中国草药对生热的影响。确认效果后，使用HPLC-Q-TOF/MS鉴定肉桂成分，并通过数据库进行筛选。组件的目标是通过TCMSP，SymMap，Swiss和STITCH数据库获得的。用DisGeNET和GeneCards数据库预测生热基因。蛋白质-蛋白质相互作用网络是用Cytoscape 3.7.1软件构建的。GO富集分析是通过STRING数据库完成的。KEGG通路分析是使用Omicshare工具建立的。根据PPI网络的边数获得了四种化合物的前20个目标。此外，网络结果已通过实验研究验证了提取物和主要化合物的作用。

结果

肉桂提取物显著上调了冷暴露过程中的体温。HPLC-Q-TOF/ MS中鉴定出121种成分。其中，数据库中包含60种化合物。获得了该化合物的116个靶标，并且41个基因与生热有关。网络结果揭示了27种有效成分和39种靶基因。通过KEGG分析，最主要的3个途径是PPAR信号传导途径，AMPK信号传导途径，生热途径。产热蛋白PPARγ，UCP1和PGC1-α被包括在四种主要化合物的关键目标中。这三种主要化合物增加了脂质的消耗并激活了褐色脂肪细胞。他们还上调了UCP1，PGC1-α和pHSL的表达，尤其是首次证实了2-甲氧基肉桂醛的作用。此外，肉桂醛和肉桂提取物还激活了TRPA1在DRG细胞上的表达。

结论

通过网络药理和实验验证研究了肉桂对抗寒性的作用机理。这项工作为支持热生成的传统应用提供了研究方向。

（主要看原文表达，有助于我们自行撰写SCI）

图文摘要

亮点

•用HPLC-Q-TOF / MS分析肉桂提取物，并用数据库筛选潜在化合物。

•通过网络药理学预测化合物的靶标和产热功能。

•对肉桂提取物及其主要化合物的产热进行了评估。

•首次检测到2-甲氧基肉桂醛的产热作用。

•抗寒作用可能与脂肪分解有关。

【结果部分】略

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