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磁流体镊钳捕获﹑操控以及结晶活体细胞

2017-01-11 RSC Publishing RSCPublishing RSCPublishing

韩国蔚山国立科技大学的Bartosz Grzybowski和同事们提出了一种利用磁性“微笔”来捕获和操控活体细胞的非侵入性、无标记镊钳法。这种方法促使规则形状的细胞群的形成,避免了诸如加热或对细胞进行磁性标记等传统镊钳法带来的问题。

Source: © Royal Society of Chemistry

Confocal images of a single cell under the magnetic micropen before and after turning on the external field

Single cell manipulation can provide insight into cell mechanics and adhesion, and has a crucial role in in vitro fertilization (IVF). Bartosz Grzybowski at Ulsan National Institute of Science and Technology in South Korea and his team’s new technique for this doesn’t need cells to be magnetically tagged beforehand. It also avoids the risks of heat- or stress-induced cell damage that can occur with other methods.

Grzybowski et al.’s method relies on an iron oxide nanoparticle medium in which cells are suspended. Applying an electromagnet to the magnetic medium through a micropen creates field gradients, which direct the cell to move in a certain direction. By varying how the micropen “tweezers” are positioned, cell movement can be manipulated in both 2 and 3 dimensions.

As well as controlling a single cell, the micropen can be used to pick up several cells together and guide them into regularly shaped clusters. Although it’s a long way off, this could one day be used to make IVF processes more efficient, reducing the number of potential embryos that need to be discarded. It could also be extended to manipulating bacteria and other single-celled organisms to conduct detailed studies on their behaviour.

Read abstract of this article:

Trapping, manipulation and crystallization of live cells using magnetofluidic tweezers
J. V. I. Timonen, C. Raimondo, D. Pilans, P. P. Pillai and B. A. Grzybowski*

Nanoscale Horiz., 2017, 2, 50-54

DOI: 10.1039/C6NH00104A

Live mammalian cells are captured and manipulated in magnetofluidic traps created in a suspension of biocompatible, magnetic nanoparticles by a coaxial magnetic/non-magnetic “micropen”. Upon activation by an external electromagnet, the pen creates microscale gradients of magnetic field and nanoparticle concentration that translate into directional and confining forces acting on the cells. Both individual cells and cell collections can be trapped by this method, allowing, for instance, for the formation of regularly shaped cell assemblies. The method does not entail any local heating artifacts and does not require magnetic tagging of the cells.

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