在本期内容里，我们继续挑选了近期2篇已发表文章的部分审稿意见，根据审稿人提出的问题分为补充实验、结果质疑、写作问题和图表问题。一起看看作者是怎么回复的吧 ~
文章题目：Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activation审稿人2
在这些实验中，CD107细胞的百分比变异性很高……作者应该在图4E中包括所有4个小鼠组。图4E和4F中使用的样本缺乏标签，使得图片看起来难以理解。We agree with Reviewer #2 comment on CD107…… As Reviewer #2 noticed, in the figure 4D the lower levels for CD107 were chosen for illustration of all conditions to be constant. To remove any concerns due to the variable expression of CD107, we performed another set of experiments. Reformated figure 4D with points close to the average value for each conditions……我们同意审稿人2对CD107的评论……正如他所注意到的，在图4D中，较低的CD107水平是为了说明所有的条件是恒定的。我们进行了另一组实验，以消除由于CD107的变异表达而产生的担忧。重新制作的图4D中的点接近每个条件的平均值……We apologize for the absence of clear labelling of 4E and 4F. This point has been fixed in the revised version of the manuscript of the figure 4 legend.我们对图4E和4F没有明确的标注表示歉意。这一点已经在修订稿中图4的图注中修改。文章题目：Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases审稿人2
使用……评估了体内治疗效果……并未在小鼠模型的治疗效果方面进行评估。强烈建议包括 NIPP1-CD 数据，否则，这项研究所做的工作太浅了。此外，在非常小的动物样本中评估了 CT-Casp-3……作者应该在较大的动物样本基础上进行这项实验。To address these concerns we have performed additional experiments and included more data in the manuscript. We repeated the efficacy experiment with a second liver cancer model……After ten days, CT Casp-3 Salmonella reduced tumor volume by about 50% ……we have added the new results as Figure 7H and added text to the caption to Figure 7……为了解决这些问题，我们已经进行了额外的实验，并在稿件中加入了更多的数据。我们用第二个肝癌模型重复了疗效实验……十天后，CT Casp-3沙门氏菌使肿瘤体积减少了大约50%……我们已经将新的结果添加到图7H中……We have also added results showing the efficacy of NIPP1-CD Salmonella in tumor-bearing BALB/c mice to the Supplemental information (Figure S2)……This shows that the efficacy of a bacterial delivered protein is dependent on many factors, more than just cellular cytotoxicity. To address this point, text was added to the……我们还在补充信息中添加了显示 NIPP1-CD 沙门氏菌在荷瘤 BALB/c 小鼠中的疗效的结果……这表明，细菌递送蛋白质的效果取决于许多因素，而不仅仅是细胞毒性。为了解决这个问题，在……中增加了内容。文章题目：Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activation审稿人2
图5D中，CD107水平和阳性T细胞的百分比要比以前的实验低得多。这仅仅是由于CD107 染色/门控的变化，还是模型中T细胞的激活存在本质区别？Compare to other figures, in Fig 5D the tumor cell lines we used are different……We agree with the Reviewer #2 that ……与其他图相比，在图5D中我们使用的肿瘤细胞系是不同的……我们同意审稿人#2的观点……This can be due to cell line variability of immunogenicity after the introduction of the molecular construct. ……Given that CD107 expression at the cell surface is extremely labile……the weak surface expression of CD107 can be explained by the exacerbated activation of……这可能是由于引入分子结构后，细胞系免疫原性的变异……鉴于CD107在细胞表面的表达是非常不稳定的……所以CD107的表面低表达可以解释为……的激活加剧了。Though the description of …… was depicted in the methods, to avoid reader confusion with …… we added this comment in the figure legend of Fig5虽然……是在方法中描述的，为了避免读者混淆……我们在图5的图注中加入了这个内容。文章题目：Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases审稿人1
……作者声称“递送蛋白质有效载荷到癌细胞中”、“只在肿瘤细胞释放蛋白”和“安全且具有自限性”，从而夸大了这项工作。此外，由于 ID 沙门氏菌只能延缓肿瘤生长，应避免使用“消除实体瘤”和类似的描述。The reviewer raises important concerns about……and language in the manuscript. To address the concern about toxicity, we performed additional experiments to……审稿人对……和手稿语言问题提出了重要的建议。为了解决对毒性的担忧，我们进行了额外的实验……As suggested by the reviewer, we have also changed language throughout the manuscript. We have removed the word “eliminated” from the …… We have also made all of the suggested changes to remove claims of ……. Text was changed in the……根据审稿人的建议，我们还更改了整个手稿的语言。我们已从……中删除了“eliminated”一词。我们还对所有建议进行了更改，删除了有关……的表述，更改的地方在……文章题目：Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activation审稿人2
图1B,D,F使用了饼状图——这些图在这里没有帮助，因为没有提供任何关于肿瘤之间变化的指示……We appreciate Reviewer #2 suggestion. In figure 1B, D, F of the revised manuscript, pie charts have been replaced by histogram bars showing all individual points per tumor我们感谢审稿人#2的建议。在修订稿的图1B、D、F中，饼状图已改为能够显示了每个肿瘤所有单个数据点的直方图。修改后的图1bdf
文章信息：
[1] Lainé, A., Labiad, O., Hernandez-Vargas, H. et al. Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activation. Nat Commun 12, 6228 (2021). https://doi.org/10.1038/s41467-021-26352-2
[2] Raman, V., Van Dessel, N., Hall, C.L. et al. Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases. Nat Commun12, 6116 (2021). https://doi.org/10.1038/s41467-021-26367-9
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