【期刊】《分析化学》2020年第7期英文版正式发布
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Recent Progress on Tissue Analysis by Mass Spectrometry without Sample Pretreatment
WANG Yu-Fen1, LU Hai-Yan1, ZHANG Hua1,2, CHEN Huan-Wen2,*
1 State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, China2 Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China无需样品预处理直接对组织样品进行质谱分析的技术获得了广泛关注。本文综述了大气压离子质谱分析技术在植物、动物和临床组织样品分析中的应用进展，总结了对各类代表性样品无需预处理进行直接质谱分析或质谱成像的方法特点及发展趋势。
Abstract
Tissue samples are widely used for the study of metabolomics, lipidomics and proteomics due to their abundant useful biomolecular information. Modern analytical mass spectrometry has the ability to directly obtain the molecular information contained in various tissue samples (e.g., plant tissues, animal tissues, and clinical tissues, etc.) in real-time, with minimal or no sample pretreatment. Such features of analytical mass spectrometry promote the throughput of tissue analysis and extend the application of tissue analysis in multiple fields. This review summarizes the applications of mass spectrometry in direct analysis of plant tissues, animal tissues and clinical tissues, and the challenges and perspectives of mass spectrometry in direct analysis of tissue samples are discussed as well.
Graphical Abstract
This review summarizes the applications of ambient ionization mass spectrometry and imaging techniques in direct analysis of plant tissues, animal tissues and clinical tissues, showing its potential applications in the fields of disease diagnosis, drug research, environmental monitoring, food safety, agricultural production and biomimetic materials.
Imaging of Cell Migration Mediated Exocytosis with Gold Nanoprobes
XIE Xiao-Dong1,2, YIN Min3, LI Qian4, CHEN Nan3,*
暗场显微成像技术可以对活细胞内等离子体探针的分布、运动和聚集等进行实时成像。本研究采用AuNPs 作为暗场成像探针,借助单颗粒示踪的方法研究了细胞迁移过程中AuNPs 的运动和外排；首次证明了纳米颗粒可通过细胞迁移介导的方式被排出细胞外,该发现对于设计安全高效的纳米载体具有指导意义。
Abstract
Insight into the exocytosis process of diagnostical nanomaterials is critical for their clinical transformation. However, it is unclear whether nanomaterials will be eliminated during cell migration. Herein, gold nanoparticles (AuNPs) were utilized as plasmonic imaging probes and dark-field microscopy was applied to examine the movement as well as exocytosis of AuNPs during cell migration. The results showed that internalized AuNPs could localize into the retraction fibers. The mobility of nanoparticles reduced with the increased distance from the cell body. When the retraction fibers disconnected from the cell body, AuNPs were left outside the cell. This study demonstrated the migracytosis of nanomaterials, which could benefit the future design of safer and more efficient nanomaterials for diagnostics.
Graphical Abstract
The distribution and movement of plasmonic gold nanoparticles (AuNPs) during cell migration was investigated by dark-field imaging and single particle tracking. The results demonstrated that nanoparticles could be expelled via cell migration mediated exocytosis. This information could benefit the future design of safer and more efficient nanoreagents for biomedical application.
Accurate Determination and Characterization of Gold Nanoparticles Based on Single Particle-Inductively Coupled Plasma-Mass Spectrometry
CHAO Jing-Bo1,*, WANG Jing-Ru1, ZHANG Jing-Qi1,2
1 Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China
2 Chemical Engineering School, Beijing University of Chemical Technology, Beijing 100029, China
建立SP-ICP-MS检测纳米颗粒数量浓度和粒径的方法，其中传输效率对颗粒数量浓度的检测结果的准确度影响较大。本文分别以数量浓度计算传输效率检测颗粒数量浓度、以粒径计算传输效率测量颗粒尺寸，结果与理论值偏差小于1.5%，并在测定过程中插入校准标准以校正传输效率的波动，为保证结果的准确性需在不同驻留时间下选择合适的数据处理模式。通过合成样品和 CCQM 国际比对样品的测定验证其准确性和适用性，国际比对颗粒数量浓度结果与关键比对参考值吻合，测定结果等效一致，扩展不确定度<15%(k=2)。
Abstract
An accurate determination method for gold nanoparticles (AuNPs) number concentration and size was developed based on single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS). Influencing parameters such as different calculation methods of transport efficiency, dwell time, dispersing agent and injection mode were investigated and optimized. The transport efficiency has a greater impact on the accuracy of the measurement result, and it should be selected according to the measured target parameters. It was important to keep the stability of the transport efficiency and use the calibration standard calibrating during the measurement process. The dwell time directly affected the precision of result, and the data processing mode should be selected carefully according to the sample type, particle size and other properties. When the dwell time was 0.1 ms, the linear ranges of 30 nm and 60 nm AuNPs were 2–40 pg/g and 10–200 pg/g, respectively, the linear correlation coefficient was greater than 0.9998, the relative standard deviation was less than 3.80%. The detection limits of particle number concentration and particle size were 110 NPs/g and 9 nm, respectively. Four kinds of synthesized AuNPs and international pilot study sample were determined by the established method. The deviation of the number concentration in pilot study from the key comparison reference values was less than 5%. The results were equivalent and the expanded uncertainty was less than 15% (k = 2), which further verified the accuracy and applicability of the method.
Graphical Abstract
The accuracy and stability of transport efficiency have a great influence on the results of particle number concentration and particle size. When transport efficiency was calibrated by particle number, the result of particle number concentration was closer to the theoretical calculated value. The result of particle size was more accurate when the transport efficiency was calibrated by particle size. The calibration standard was inserted in the determination process to prevent the fluctuation of transport efficiency. In order to ensure the accuracy of the results, the appropriate data processing mode is selected according to different dwell time.
Stimuli-responsive Polymers-based Two-dimensional Photonic Crystals Biosensors
CUI Chun-Guo1, YOU Ai-Mei2, SHI Kai-Yao3,*, LIU RUI4, LU Yu-Yuan5, ZHANG Qiang2,*
1 Department of Breast Surgery, the Third Hospital of Jilin University, Changchun 130033, China
2 State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
3 Provincial Key Laboratory for Gene Diagnosis of Cardiovascular Disease, Jilin Provincial Engineering Laboratory for Endothelial Function and Genetic Diagnosis, Department of Cardiology, the Third Hospital of Jilin University, Changchun 130033, China
4 Drug Engineering Research Center, Harbin University of Commerce, Harbin 150076, China
5 State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
光子晶体是一种介电常数在空间呈周期性变化的功能材料，由于在光、电、热、磁学等方面具有优良的性能成为目前研究的热点。基于刺激响应性聚合物的二维光子晶体主要由响应性水凝胶和组装微粒两部分组成。微粒在水凝胶表面自组装形成二维有序结构，并呈现出结构色，根据结构色的变化可以实现对各种分析物的检测。本文综述了二维光子晶体制备方法及在生物传感检测方面的应用。
Abstract
Stimuli-responsive polymers-based two-dimensional photonic crystals (2DPCs) are comprised of two main components, stimuli-responsive hydrogels and structured microparticles. Microparticles can self-assemble into ordered structures in two dimensional spacing on the surface of hydrogels. The ordered structure allows structural colors that can be used as indicator phenomena to detect various analytes. The changes in colors are associated with the concentration change of analytes. Therefore, the concentration of the analytes can be determined using a simple colorimetric comparison. 2DPCs have attracted much attention due to their sensing applications. This article covers a brief general introduction of 2DPC and highlights many milestone examples as biosensors to inspire more innovative research work in the near future.
Graphical Abstract
Two-dimensional photonic crystal (2DPC) sensors are constructed by assembling the monolayer ordered building blocks on the surface of the stimuli-responsive hydrogels. When analyte presents in the system, it will selectively bind with the functional hydrogels, which will make hydrogel swell or shrink, in turn changing the interparticle distance. The amount of the analyte in the system will be directly related to the color changes observed and thus the analyte concentration could be determined using a simple colorimetric comparison method.
Phenothiazine and BN-doped AIE Probes Integrated Fluorescence Sensor Array for Detection and Discrimination of Nitro Explosives
CHENG Cheng1,2, NAWAZ Muhammad-Azhar-Hayat2,3, LIU Chao-Yi2, SHAHZAD Sohail-Anjum4, ZHOU Hui-Peng2,*, YU Cong2,3, JIN Xing1,*
1 School of Chemistry and Pharmacy, Jilin Institute of Chemical Technology, Jilin 132022, China
2 State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
3 University of Science and Technology of China, Hefei 230026, China
4 Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan
本研究合成了一种新型的AIE探针SN-1，并将其用于爆炸物的检测。将吩噻嗪类探针SN-2与AIE探针SN-1进行有机组合，成功构建了一种荧光传感器阵列，并实现了对五种硝基炸药的鉴别。硝基炸药对探针SN-1和SN-2的响应浓度低至nmol/L水平。此探针是一种简单，稳定和可靠的荧光传感器阵列。
Abstract
Highly effective sensing of nitro explosives plays an extremely important role in homeland security and counter-terrorism. In this work, a BN-doped aggregation induced emission (AIE) probe SN-1 was synthesized and subsequently characterized. The probe was then exposed to DNT and TNT, which resulted in formation of intermolecular hydrogen bonds between the probe and the two nitro explosives, and thus fluorescence quenching response was achieved. In addition, an electron rich phenothiazine probe SN-2 was also prepared to probe the response to the nitro explosives via photo-induced electron transfer. The two probes were combined to construct a fluorescence sensor array for the successful distinction of five nitro explosives. This reliable and sensitive fluorescence sensor array held a good potential for homeland security and environmental management.
Graphical Abstract
In this work, fluorescence probes SN-1 and SN-2 were designed and synthesized. They showed distinct respond to nitro explosives, and were subsequently used to construct a fluorescence sensor array to successfully distinguish five nitro explosives.
《分析化学》创刊于1972年，由中国科学院长春应用化学研究所和中国化学会共同主办，报道我国分析化学创新性研究成果，反映国内外分析化学学科的前沿和进展。主要栏目包括特约来稿、研究快报、研究报告、仪器装置与实验技术、评述与进展等。现任名誉主编汪尔康院士，主编杨秀荣院士。《分析化学》期刊于1999年被SCI-E收录，2006年被Scopus, 2016年被EI收录。《分析化学》始终致力于打造国际知名学术期刊，以创办中文精品期刊为目标；同时，《分析化学》与国际著名科技出版商Elsevier公司合作，发行本刊的英文电子版
（www.sciencedirect.com/journal/chinese-journal-of-analytical-chemistry, ISSN1872-2040）。
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