【直播】【光讲】基于数字全息的组织和细胞定量相位成像

活动名称：

光讲线上学术论坛 | Quantitative phase imaging with digital holographic microscopy for cytometry and histology

活动时间：

2022年10月9日（周日）19:00

报告嘉宾：

Prof. Björn Kemper

主办单位：

中国科学院西安光学精密机械研究所

直播通道

蔻享学术直播间 识别二维码，即可观看直播。

报告人介绍

Prof. Björn Kemper

Biomedical Technology Center, University of Muenster, Germany

Prof. Björn Kemper, Head of Optical Technologies, Biomedical Technology Center, University of Muenster, Germany. Prof. Björn Kemper received his Ph.D. in physics from the Humboldt Universität (Berlin, Germany) in 2001. He is an internationally recognized researcher in the field of label-free imaging with coherent and partial coherent light, digital holographic endoscopy, digital holographic microscopy (DHM), quantitative phase imaging (QPI) and the application of DHM and QPI for quantitative live cell and tissue analysis. Prof. Kemper has developed several DHM systems for various applications in cytometry of living cells, tissue analysis and histopathology. He is author and co-author of more than 200 publications including articles in peer reviewed journals, books, and conference proceedings, is reviewer for various national and international funding organizations and peer reviewed scientific journals, guest editor of special issues in peer reviewed journals and member of the program committees of several scientific conferences. In 2018 he was awarded as guest professor of the Chinese Academy of Science.

报告简介

Within the last decade, quantitative phase imaging (QPI) was continuously improved for high resolution label-free quantitative microscopy. Digital holographic microscopy (DHM) is an interferometry-based variant of QPI that typically utilizes a laser as coherent light source. DHM can be modularly integrated into common optical microscopes and provides quantitative phase contrast by detection of specimen induced optical path length changes against the surrounding environment. The reconstruction of digital holograms is performed numerically. Thus, multi focus imaging of specimen and subsequent autofocusing from single captured digital holograms without mechanical focus realignment are achieved. In transmission configuration DHM requires only low light intensities for object illumination and thus minimized the interaction with the investigated sample, which is essential for minimally invasive long-term monitoring of living cell cultures. Moreover, DHM-based phase contrast imaging simplifies automated object tracking and image segmentation for quantification of cell migration and morphology by extraction of absolute biophysical parameters such as cell volume, thickness, or dry mass. In addition, QPI with DHM provides access to the cellular refractive index which is related to the cellular water content, intracellular solute concentrations, and tissue density. After an introduction to QPI with DHM, in an overview, representative applications are presented. First, capabilities of DHM to determine cell motility and growth dynamics during in vitro drug and toxicity testing are illustrated. Then, briefly the potential in histology is demonstrated by quantification of inflammation and nanomaterial induced pathological alterations in ex vivo colon and lung tissue sections. Finally, results from holographic image flow cytometry and perioperative monitoring of living primary human leucocytes after cardiac surgery prospect DHM as a cytometric tool for temporal disease course characterization.

“光讲”栏目简介

1、栏目背景

光讲有用--传播有用的光学知识。

由两江科技评论与中科院青促会西安光机所小组联手打造，依托新媒体的服务性、指导性和公开性，致力于传播科技理念与科技成果，促进科研工作者快速了解光学前沿资讯。旨在起到引领科研，传递知识的作用。

2、栏目内容

邀请国内外院士、诺奖获得者、领域知名科学家、中青年科学家等围绕物理前沿、光学领域等相关研究进展及最新研究成果进行线上报告，打造高端、前沿、严谨的线上光学论坛，传播光学知识，扩大研究成果影响力，促使光学在科技前沿、社会发展与国民生产中发挥更大作用。

两江科技评论

析光学术

---

推荐阅读

编辑：黄琦

蔻享学术 平台介绍

蔻享学术平台，国内领先的一站式科学资源共享平台，依托国内外一流科研院所、高等院校和企业的科研力量，聚焦前沿科学，以优化科研创新环境、传播和服务科学、促进学科交叉融合为宗旨，打造优质学术资源的共享数据平台。

识别二维码，

下载 蔻享APP  查看最新资源数据。

点击阅读原文，查看更多精彩报告！