中科大何立群等 | 基于微流控输运网络节点阻力特征的微流控芯片精准设计方法

内容简介

本研究论文聚焦基于微流控输运网络节点阻力特征的微流控芯片精准设计方法。微流控通道内流体的流动特征为层流，故通道内压降与流量呈线性相关，其比值称为阻力，取决于通道的尺寸与动力粘度。通常，微流控芯片设计方法类似于电路设计，主要关注直管段尺寸布置。其实，水力网络设计与电路设计略有不同。体现在节点上，电路局部电压损失可以忽略不计，但流体局部压降相对于直管段压降不能忽略。本文着重解决常见节点局部阻力计算方法，通过数值计算建立了微流体局部阻力构件库。据此，建立了模块化的微流控芯片设计方法。我们设计几组实验检验所建立的方法。其中，通过阿霉素刺激内皮细胞后观察和分析内皮细胞的反应来进行最终的判断。结果表明，考虑局部阻力后，微流控芯片分配组分的精度获得极大提高，表明在设计中考虑局部阻力是十分必要的。本文所建立的设计方针有助于微流控芯片更加准确地用于单分子水平的分析与检测，甚至设计更为精准的器官芯片。

引用本文（点击最下方阅读原文可下载PDF）

Lin Y, He D, Wu Z, et al., 2022. Junction matters in hydraulic circuit bio-design of microfluidics. Bio-des Manuf (Early Access). https://doi.org/10.1007/s42242-022-00215-1

文章导读

图1 微流控芯片设计和制造过程

图2 实验系统搭建

图3 双聚焦微流控芯片用于单细胞分析的精准设计

图4 染料（试剂）在浓度梯度网络的微流控芯片中输运

图5 通过输运网络产生设计的均匀浓度阿霉素来刺激内皮细胞

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