纳/微米材料及器件的生物医学应用虚拟专辑代表性论文9： 阳离子多赖氨酸修饰磁性氧化铁纳米粒颗粒用于肺癌细胞高效标记

【引言】

Scheme 1 Schematic representation of the PLL-MNP preparation and delivery to human A549 lung cancer cells

摘要

磁性氧化铁纳米颗粒标靶癌细胞已成为癌症的细胞疗法的常规手段之一。河南科技大学的Wang Xueqin首次合成了磁性γ-Fe2O3，通过阳离子多赖氨酸表面修饰成功实现对人类肺癌细胞 (A549)的磁性标记。在低浓度下，细胞的存活率、增殖能力、细胞周期与凋亡率未受影响。此外，与未处理细胞相比，处理过的细胞骨架形态保持更为完整。在高浓度下(400 ug mL-1), 多赖氨酸修饰磁性γ-Fe2O3纳米颗粒会轻微损害细胞的存活率、增殖能力、骨架形态等。本研究表面，在适当浓度下，多赖氨酸修饰磁性γ-Fe2O3纳米颗粒可作为A549肺癌细胞的有效标靶物，并在磁性靶向抗癌药物/基因递送，靶向诊断和治疗肺癌细胞等方面具有 较好的应用前景。

这篇文章发表于Nano-Micro Letters上2015年第7卷第4期. 

全文链接：10.1007/s40820-015-0053-5
文章引用信息：

Xueqin Wang . Huiru Zhang . Hongjuan Jing . Liuqing Cui,Highly Efficient Labeling of Human Lung Cancer Cells Using Cationic Poly-L-lysine-Assisted Magnetic Iron Oxide Nanoparticles,Nano-Micro Lett. (2015) 7(4):374–384, http://dx.doi:10.1007/s40820-015-0053-5

【图文导读】

Fig. 1 (a) TEM image, (b) EDX results, and (c) XRD pattern of MNPs.(d) TEM image of PLL-MNPs

Fig. 2 (a) FT-IR spectra of MNPs (top), PLL-MNPs (middle), and pure PLL (bottom). (b) The magnetic loop of PLL-MNPs at 300 K

Fig. 3 Prussian blue staining of A549 lung cancer cells labeled with PLL-MNPs at different concentrations (25, 50, 100, 200, and 400 lg mL-1 for (a), (b), (c), (d), and (e), respectively) and( f) unlabeled A549 cells used as controls. Scale bar 100 lm

Fig. 4 The temporal growth state of the PLL-MNP-labeled A549 lung cancer cells incubated in RPMI-1640 medium, and the untreated counterparts were used as controls

Fig. 5 Cellular viability detection of the PLL-MNP-labeled A549 lung cancer cells using PI and FDA double staining. (a) Untreated A549 cells were used as controls. (b)–(f) Fluorescence images illustrated the A549 cells treated with various concentrations of PLL-MNPs (25, 50, 100, 200, and 400 lg mL-1 ) for 48 h. Scale bar 100 lm

Fig. 6 Immunofluorescent staining of the actin cytoskeleton of the PLL-MNP-labeled A549 lung cancer cells. (a) Untreated A549 cells were used as controls. (b)–(f) The A549 cells treated with different concentrations of PLL-MNPs (25, 50, 100, 200, and 400 lg mL-1 ) were cultured for 48 h in an RPMI-1640 medium. Cell nucleus was located by counterstaining with DAPI (blue). Scale bar 100 lm. (Color figure online)

Fig. 7 (a) Cell cycle distribution of the examined cells. Unlabeled A549 cells were used as controls. (b)–(f) The A549 cells labeled with different concentrations of PLL-MNPs (25, 50, 100, 200, and 400 lg mL-1 ) were cultured for 48 h

Fig. 8 Fluorescent staining of PLL-MNP-treated A549 lung cancer cells.(a) Untreated A549 cells were used as controls. (b)–(f )Fluorescence images illustrated the A549 cells treated with different concentrations of PLL-MNPs (25, 50, 100, 200, and 400 lg mL-1 ). Scale bar 100 lm

虚拟专辑（二）纳/微米材料及器件的生物医学应用

本期虚拟专辑主要介绍纳米材料及器件在生物医学领域的研究，选自近两年发表在Nano-Micro Letters上的9篇代表性论文，敬请阅读并复制链接打开下载（免费），并欢迎投稿。

代表性论文

⊙ 1. 综述：微流体在乳腺癌诊断方面的应用

链接

⊙ 2. 综述：电化学纳米医学传感器在生物医学领域的应用展望

链接

⊙ 3. 综述：ZnO纳米颗粒的抗菌活性和毒性机制

链接

⊙ 4. 金纳米颗粒单层和多层细胞模型在纳米-微界面上相互作用的尺寸依赖性

链接

⊙ 5. 具有高抗菌抗癌特性的碳纳米管内嵌植物化学官能化Cu/Ag纳米颗粒复合物

 链接

⊙ 6. 牛血清白蛋白耦联磁性Fe3O4 纳米颗粒提高生物相容性与磁热疗性能

链接

⊙ 7. 细胞在阳极氧化的多尺寸二氧化钛纳米管阵列表面的行为研究

链接

⊙ 8.利用多层细胞培养模型揭示纳米颗粒在实体肿瘤中的吸收和分布

链接

⊙ 9. 阳离子多赖氨酸修饰磁性氧化铁纳米粒颗粒用于肺癌细胞高效标记

 链接10.1007/s40820-015-0053-5

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