晚发性败血症早产儿的菌群特征

这是对患晚发性败血症的早产新生儿的肠道菌群研究，结果很有参考价值，特别推荐！

论文摘要

Late onset sepsis (LOS; defined as sepsis after 72 h of life) remains a serious and common complication of prematurity, with rates of 20–40% for infants <32 weeks gestation reported in some studies. LOS in preterm infants impacts negatively on survival (with mortality rates of up to 10%) and on developmental outcomes [1]. Mechanisms of LOS pathogenesis are poorly understood, but bacterial colonisation and low gestational age are key risk factors [2]. Bacterial profiling studies have shown that LOS infants have an altered microbiome and lower bacterial diversity [3–8], and the bacterial strain isolated in diagnostic blood culture is frequently present in the gut [9]. Central to LOS pathogenesis are bacterial-host interactions modulating gut and systemic immune responses, tight junction integrity, and host metabolic function [10]. The most common organisms causing LOS in preterm infants include coagulase-negative Staphylococcus, Escherichia, Klebsiella, and Enterococcus [11].

Recent advances in ultra-performance liquid chromato graphy-mass spectrometry (UPLC-MS) untargeted metabolomics facilitate further understanding of these complex relationships involving host and bacteria, and the complex interactions of immune and metabolic function in relation to health and disease states [12]. While largely pilot in nature, existing metabolomic studies in preterm infants have demonstrated important findings. In necrotizing enterocolitis (NEC), the most prevalent serious preterm disease after LOS, the metabolite profiles are different at diagnosis compared to controls in serum [13–15], urine [16], and stool [17]. Stool volatile organic compound and serum UPLC-MS has also demonstrated differences between LOS infants and matched controls at or immediately prior to disease [13, 15, 18]. Stool metabolite profiles are also significantly associated with age [19] and serum metabolite profiles between preterm and term neonates also differ [14]. We aimed to explore relationships between gut microbiome and metabolome to determine key insights into LOS development, impact, and recovery. This is the first study to employ UPLC-MS untargeted metabolomics of stool to determine host and bacterial functioning within the gut of infants diagnosed with LOS.

实验综述

① 对7名患晚发性败血症（LOS）的早产新生儿及28名健康对照的粪便样本进行分析；

② LOS的血液培养中分离出的细菌通常与肠道菌群中的优势菌属一致；

③ 长期监测早产儿肠道菌群群落类型（PGCT）发现，对照组婴儿PGCT数量上升，双歧杆菌为优势菌的PGCT6型菌群仅在对照组出现；

④ 诊断时和7天后LOS和对照组婴儿间代谢产物有差异，双歧杆菌与对照组代谢产物（棉子糖、蔗糖、乙酸等）正相关；

⑤ 早产婴儿肠道的发育和保护与低聚糖和有益细菌的上升相关。