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最近我们公告了《2021生信学习班起航,先送福利》,很多朋友咨询生物信息方面的书籍,这个真的好难推荐啊!我甚至都忘记了自己还有一本生物信息学的书,见http://www.biotrainee.com/jmzeng/book/basic/,因为入门生物信息学的路实在是太多了,不同的ngs数据处理都会涵盖生物信息学的编程和统计学基础。我在B站免费NGS数据处理视频课程就很多了,已经组建了微信交流群的有下面这些:

每个视频课程都对应了很多国内外生信学习资源,比如《WES数据分析》就是注重于质控比对找变异,跟另外一个课程类似:https://wikis.utexas.edu/

目录结构

Day 1 - Intro to NGS, Linux and TACC

  • Introduction
  • Getting started at TACC

Day 2: Transferring files and TACC

  • File systems and transferring files
  • Running batch jobs at TACC

Day 3: Working with raw sequences

  • Working with FASTQ files
  • Pre-processing raw sequences

Day 4: Alignment and BAM file manipulation

  • Alignment workflow

Day 5: Post-Alignment Analysis

  • Filtering with SAMTools
  • Analysis using BEDTools

Resources

Extras

  • Obtaining public datasets from NCBI
  • Visualize mapped data at UCSC genome browser
  • Shell scripting
  • Exercise solutions
  • Catch-up
  • Old stuff
  • Alignment (archive)
  • More Alignment exercises
  • Copy of good Alignment workflow
  • Samtools: viewing, counting and sorting your alignment data
  • Bedtools: Analyzing your aligned experiment
  • Piping and redirection
  • SAM format and samtools
  • Scott's Linux one-liners
  • Using TACC's Stampede cluster
  • Analysis with bedtools


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