其他
最近我们公告了《2021生信学习班起航,先送福利》,很多朋友咨询生物信息方面的书籍,这个真的好难推荐啊!我甚至都忘记了自己还有一本生物信息学的书,见http://www.biotrainee.com/jmzeng/book/basic/,因为入门生物信息学的路实在是太多了,不同的ngs数据处理都会涵盖生物信息学的编程和统计学基础。我在B站免费NGS数据处理视频课程就很多了,已经组建了微信交流群的有下面这些:
免费视频课程《RNA-seq数据分析》 免费视频课程《WES数据分析》 免费视频课程《ChIP-seq数据分析》 免费视频课程《ATAC-seq数据分析》 免费视频课程《TCGA数据库分析实战》 免费视频课程《甲基化芯片数据分析》 免费视频课程《影像组学教学》 免费视频课程《LncRNA-seq数据》 免费视频课程《GEO数据挖掘》 肿瘤基因测序
每个视频课程都对应了很多国内外生信学习资源,比如《WES数据分析》就是注重于质控比对找变异,跟另外一个课程类似:https://wikis.utexas.edu/
目录结构
Day 1 - Intro to NGS, Linux and TACC
Introduction Getting started at TACC
Day 2: Transferring files and TACC
File systems and transferring files Running batch jobs at TACC
Day 3: Working with raw sequences
Working with FASTQ files Pre-processing raw sequences
Day 4: Alignment and BAM file manipulation
Alignment workflow
Day 5: Post-Alignment Analysis
Filtering with SAMTools Analysis using BEDTools
Resources
Extras
Obtaining public datasets from NCBI Visualize mapped data at UCSC genome browser Shell scripting Exercise solutions Catch-up Old stuff Alignment (archive) More Alignment exercises Copy of good Alignment workflow Samtools: viewing, counting and sorting your alignment data Bedtools: Analyzing your aligned experiment Piping and redirection SAM format and samtools Scott's Linux one-liners Using TACC's Stampede cluster Analysis with bedtools