【直播】How a single mutation causes thesystemic disease Cystic...
本次报告由北大-清华 生命科学联合中心主办，于2021年6月3日10:00开始，授权蔻享学术进行网络直播。
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How a single mutation causes thesystemic disease Cystic Fibrosis
报告人
Prof.JohnR. Yates III
时间
Jun. 3th, 10:00-12:00 AM
A component to understanding biological processes involves identifying the proteins expressed in cells as well as their modifications and the dynamics of processes. This process has benefited from the sequencing of genomes, although this information is not uncovered from DNA sequencing. Mass spectrometry together with informatic tools can uncover the type of modification and it’s a location in a peptide sequence. Advances in multi-dimensional separations as well as mass spectrometry have improved the scale of experiments for protein identification. This has improved the analysis of protein complexes, and more complicated protein mixtures. Quantitative mass spectrometry has also helped to determine the role of modifications in regulating biological processes. Using the loss of function mutant form of the Cystic Fibrosis Transport Regulator (∆F508) as it progresses through the folding pathway as a model to understand the regulation of protein maturation, we have discovered a post translational modification code that regulates the maturation of CFTR. This has provided a better understanding of the loss of function associated with mutation. We recently developed a protein footprinting method that allows quantitative assessment of in vivo protein folding. By using this method, recent in vitro structures and chemical tools we are developing a model for how DF508 CFTR misfolds.
编辑：黄琦
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